Evaluation of a Treponema pallidum western immunoblot assay as a confirmatory test for syphilis
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چکیده
منابع مشابه
Evaluation of a Treponema pallidum enzyme immunoassay as a screening test for syphilis.
The CAPTIA Syphilis-G enzyme immunoassay for the detection of antibodies to Treponema pallidum was evaluated as a screening test for syphilis in comparison with the standard rapid plasma reagin (RPR) test. One thousand samples were tested, and the standard fluorescent treponemal antibody absorption test and the standard microhemmaglutination test were used to confirm the presence of treponemal ...
متن کاملTreponema pallidum surface immunofluorescence assay for serologic diagnosis of syphilis.
A surface immunofluorescence assay (SIFA) using live spirochetes was analyzed and compared with Western blot (WB), fluorescent treponemal antibody absorption (FTA-ABS), microhemagglutination (MHA-TP), and Treponema pallidum immobilization (TPI) assays for detecting serum antibodies to T. pallidum in patients with syphilis, in disease controls, and in healthy subjects. SIFA and WB were 99% sensi...
متن کاملQuantitative microhaemagglutination assay for Treponema pallidum antibodies in experimental syphilis.
The quantitative microhaemagglutination assay for Treponema pallidum antibodies (MHA-TP) was studies in 52 untreated and treated rabbits with experimental syphilis. Rabbits with incubating experimental syphilis were cured or inadequately treated with penicillin G and some cured rabbits were later reinfected. MHA-TP conversion occurred within 45 days in untreated rabbits. Titres reached peak lev...
متن کاملTreponema pallidum specific IgM haemagglutination test for serodiagnosis of syphilis.
The Treponema pallidum specific IgM haemagglutination (TP-IgM-HA) test uses erythrocytes sensitised with antiserum to human IgM to separate IgM from IgG in serum. Specific antitreponemal IgM captured in this way is detected by adding a second reagent comprising erythrocytes sensitised with T pallidum antigen. Eighty two serum samples from 82 patients with untreated syphilis, 521 samples from 73...
متن کاملTreponema pallidum haemagglutination test for syphilis. Evaluation of a modified micro-method.
Passive haemagglutination of erythrocytes sensitized with antigen is an extremely sensitive method for the detection of antibody. Its application to the serological diagnosis of syphilis was first reported by Rathlev (1965, 1967), using a haemagglutination technique for the detection of specific antitreponemal antibody in which formolized tanned sheep cells sensitized with ultrasonicated materi...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1992
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.30.1.115-122.1992